GSM2054693: 46c_H3K27ac_rep1; Mus musculus; ChIP-Seq - SRA

SRX1560891: GSM2054693: 46c_H3K27ac_rep1; Mus musculus; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 122.2M spots, 6.1G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: Histone H3 globular domain acetylation identifies new class of enhancers

PRJNA275703 • SRP055121 • All experiments • All runs

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We report the acetylation of lysine residues in the globular domain of H3 (H3K64ac and H3K122ac) marks active gene promoters and also a subset of active enhancers in mouse embryonic stem cells (mESCs), human erythroleukemic cell line (K562). Moreover, we find a novel class of active functional enhancers in ESCs that are marked by H3K122ac but which lack H3K27ac. This work suggests that a more complex analysis of histone acetylation is required to identify enhancers than was previously considered. Overall design: Examination of histone modifications in mouse ESCs (2 biological replicates) and K562 cells

Sample: 46c_H3K27ac_rep1

SAMN04456554 • SRS1275465 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2054693

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 122.2M spots, 6.1G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR3144859	122,158,500	6.1G	1.5Gb	2016-03-14

ID:: 2208800

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